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验证数据展示

  • IHC staining of human tonsillitis using 13071-1-AP

    IHC staining of human tonsillitis using 13071-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 13071-1-AP

    WB analysis using 13071-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 13071-1-AP

    WB analysis of HepG2 using 13071-1-AP

    WB result of CD97 antibody (13071-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD97 transfected HepG2 cells.

  • IHC staining of human tonsillitis using 13071-1-AP

    IHC staining of human tonsillitis using 13071-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CD97 Polyclonal antibody

CD97 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13071-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, CD97 antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13071-1-AP targets CD97 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3745

Product name: Recombinant human CD97 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-350 aa of BC026690

Sequence: QDSRGCARWCPQNSSCVNATACRCNPGFSSFSEIITTPTETCDDINECATPSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRFFDKVQDLGRDSKTSSAEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNLEDIMRILAKSLPKGPFTYISPSNTELTLMIQERGDKNVTMGQSSARMKLNWAVAAGAEDPGPAVAGILSIQNMTTLLANASLNLHSKKQAELEEIYESSIRGVQLRRLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD97 molecule
别名 ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, CD97 antigen
计算分子量 722 aa, 79 kDa
观测分子量 75-85 kDa
GenBank蛋白编号BC026690
基因名称 CD97
Gene ID (NCBI) 976
RRIDAB_10646477
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48960
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

实验方案

Product Specific Protocols
IHC protocol for CD97 antibody 13071-1-APDownload protocol
WB protocol for CD97 antibody 13071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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