• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • IF Staining of RAW 264.7 using 84801-4-RR

    IF Staining of RAW 264.7 using 84801-4-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD9 antibody (84801-4-RR, Clone: 242432C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse kidney using 84801-4-RR

    IHC staining of mouse kidney using 84801-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-4-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 84801-4-RR

    IHC staining of mouse kidney using 84801-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-4-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84801-4-RR

    Affinity and Kinetic Characterization of 84801-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84801-4-RR against Mouse CD9 were performed. The affinity constant is below 1 pM.

CD9 Recombinant monoclonal antibody

CD9 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84801-4-RR

产品说明书

CloneNo. 242432C2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, IF/ICC, ELISA

别名

242432C2, CD9 antigen, MIC3, P24, Tspan 29

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84801-4-RR targets CD9 in IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1397

Product name: Recombinant Mouse CD9 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 110-193 aa of NM_007657.3

Sequence: THKDEVIKELQEFYKDTYQKLRSKDEPQRETLKAIHMALDCCGIAGPLEQFISDTCPKKQLLESFQVKPCPEAISEVFNNKFHI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD9 antigen
别名 242432C2, CD9 antigen, MIC3, P24, Tspan 29
计算分子量25 kDa
GenBank蛋白编号NM_007657.3
基因名称 Cd9
Gene ID (NCBI) 12527
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP40240
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and supports myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in the fertilization of mammals (PMID: 25536312).

实验方案

Product Specific Protocols
IF protocol for CD9 antibody 84801-4-RRDownload protocol
IHC protocol for CD9 antibody 84801-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...