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验证数据展示

  • IHC staining of human tonsillitis using 86063-2-RR

    IHC staining of human tonsillitis using 86063-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human tonsillitis using 86063-2-RR

    IHC staining of human tonsillitis using 86063-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IF Staining of human tonsillitis using 86063-2-RR

    IF Staining of human tonsillitis using 86063-2-RR

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (86063-2-RR, Clone: 243018F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of human tonsillitis using 86063-2-RR

    IF Staining of human tonsillitis using 86063-2-RR

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (86063-2-RR, Clone: 243018F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86063-2-RR

    Affinity and Kinetic Characterization of 86063-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86063-2-RR against Human CD8a were performed. The affinity constant is 13.2 nM.

CD8a Recombinant monoclonal antibody

CD8a Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 86063-2-RR

产品说明书

CloneNo. 243018F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

CD8, CD8a molecule, Leu2, MAL, p32

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human ovary cancer tissue, human stomach cancer tissue, human rectal cancer tissue, human hepatocellular cancer, human breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86063-2-RR targets CD8a in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0819

Product name: Recombinant Human CD8a protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-182 aa of NM_001145873.1

Sequence: SQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD8a molecule
别名 CD8, CD8a molecule, Leu2, MAL, p32
计算分子量26 kDa
GenBank蛋白编号NM_001145873.1
基因名称 CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01732-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 22-182 aa of human CD8a, the alpha chain of CD8 molecule.

实验方案

Product Specific Protocols
IHC protocol for CD8a antibody 86063-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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