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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86063-1-RR (same clone as 86063-1-PBS)

    WB analysis using 86063-1-RR (same clone as 86063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86063-1-RR (CD8A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse thymus using 86063-1-RR (same clone as 86063-1-PBS)

    IF Staining of mouse thymus using 86063-1-RR (same clone as 86063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD8a antibody (86063-1-RR, Clone: 243018F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse thymus using 86063-1-RR (same clone as 86063-1-PBS)

    IF Staining of mouse thymus using 86063-1-RR (same clone as 86063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD8a antibody (86063-1-RR, Clone: 243018F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86063-1-PBS in a different storage buffer formulation.

CD8a Recombinant antibody, PBS Only

CD8a Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 86063-1-PBS

产品说明书

CloneNo. 243018F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, Indirect ELISA

别名

CD8, CD8a molecule, Leu2, MAL, p32

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.

产品信息

86063-1-PBS targets CD8a in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CD8a fusion protein Eg0819 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD8a molecule
别名 CD8, CD8a molecule, Leu2, MAL, p32
计算分子量26 kDa
观测分子量25 kDa
GenBank蛋白编号NM_001145873.1
基因名称 CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01732-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 22-182 aa of human CD8a, the alpha chain of CD8 molecule.