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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85977-4-RR (same clone as 85977-4-PBS)

    WB analysis using 85977-4-RR (same clone as 85977-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85977-4-RR (CD8A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85977-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse thymus using 85977-4-RR (same clone as 85977-4-PBS)

    IHC staining of mouse thymus using 85977-4-RR (same clone as 85977-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 85977-4-RR (CD8a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85977-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse thymus using 85977-4-RR (same clone as 85977-4-PBS)

    IHC staining of mouse thymus using 85977-4-RR (same clone as 85977-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 85977-4-RR (CD8a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85977-4-PBS in a different storage buffer formulation.

Mouse CD8a Recombinant antibody, PBS Only

CD8a Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85977-4-PBS

产品说明书

CloneNo. 242879E1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Indirect ELISA

别名

CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85977-4-PBS targets CD8a in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 CD8a fusion protein Eg3265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD8 antigen, alpha chain
别名 CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35
计算分子量27 kDa
观测分子量30-35 kDa
GenBank蛋白编号NM_001081110.2
基因名称 Cd8a
Gene ID (NCBI) 12525
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01731
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).