CoraLite® Plus 488 Anti-Mouse CD86 (GL1)
CD86 Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
GL1
验证数据展示
经过测试的应用
| Positive FC detected in | LPS treated C57BL/6 mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65068-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65068 targets CD86 in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC |
| Tested Reactivity | Mouse |
| Immunogen | LPS-activated CBA/Ca mouse spleen B cells 种属同源性预测 |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD86 antigen |
| Synonyms | B7, B7 2, B7.2, B70, Cd28l2, Cd86, CD86 antigen, CLS1, ETC 1, Ly 58, Ly58, MB7, MB7 2, TS/A 2 |
| GenBank Accession Number | BC013807 |
| Gene Symbol | Cd86 |
| Gene ID (NCBI) | 12524 |
| RRID | AB_2883160 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P42082 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD86 antibody CL488-65068 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
