CoraLite® Plus 488 Anti-Mouse CD86 (GL1)

CD86 Monoclonal Antibody for FC
Cat No. CL488-65068

产品说明书

CloneNo. GL1

宿主/亚型

Rat / IgG2a, kappa

种属反应性

mouse

应用

FC

Cd28l2, B7-2, B70, B7.2, B7 2

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive FC detected inLPS treated C57BL/6 mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65068-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

CL488-65068 targets CD86 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
文献引用应用FC
经测试反应性 mouse
文献引用反应性mouse
免疫原 LPS-activated CBA/Ca mouse spleen B cells 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD86 antigen
别名 Cd28l2, B7-2, B70, B7.2, B7 2
GenBank蛋白编号BC013807
基因名称 Cd86
Gene ID (NCBI) 12524
RRIDAB_2883160
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP42082
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD86 antibody CL488-65068Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

Respir Res

YTHDF1-mediated m6A modification of GBP4 promotes M1 macrophage polarization in acute lung injury

Authors - Fengan Cao
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