Anti-Human CD86 (BU63)

CD86 Monoclonal Antibody for IF/ICC, FC
Cat No. 65165-1-Ig

产品说明书

CloneNo. BU63

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human and More (1)

应用

IF/ICC, FC and More (1)

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive IF/ICC detected inRaji cells
Positive FC detected inDaudi cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65165-1-Ig targets CD86 in IF/ICC, FC applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC Application Description
文献引用应用IF, FC
经测试反应性 Human
文献引用反应性human
免疫原 B-lymphoblastoid cell line ARH 77 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD86 molecule
别名 Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72
计算分子量 329 aa, 38 kDa
GenBank蛋白编号BC040261
基因名称 CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
RRIDAB_2881481
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP42081
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

实验方案

Product Specific Protocols
IF protocol for CD86 antibody 65165-1-IgDownload protocol
FC protocol for CD86 antibody 65165-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Agric Food Chem

Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags

Authors - Zilong Zhao
humanFC

Int Immunopharmacol

Isosilybin A exhibits anti-inflammatory properties in rosacea by inhibiting MAPK pathway and M1 macrophage polarization

Authors - Chen-Chen Wu
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