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验证数据展示

  • FC experiment of Human Monocytes-derived dendritic cells using APC-65171

    FC experiment of Human Monocytes-derived dendritic cells using APC-65171

    1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 10 ul APC Anti-Human CD83 (APC-65171, Clone:HB15e) (blue) or APC mouse IgG1 isotype control (black). Cells were not fixed.

APC Anti-Human CD83 (HB15e)

CD83 Monoclonal Antibody for FC
Cat No. APC-65171

产品说明书

CloneNo. HB15e

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human, non-human primates

应用

FC

别名

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  APC
规格价格库存

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经过测试的应用

Positive FC detected inHuman monocytes-derived dendritic cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 15 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65171 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

经测试应用 FC Application Description
经测试反应性 human, non-human primates
免疫原 Human CD83-transfected Cos cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD83 molecule
别名 B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule
计算分子量 205 aa, 23 kDa
GenBank蛋白编号BC030830
基因名称 CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
RRIDAB_2918931
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
形式 Liquid
纯化方式The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDQ01151
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for APC CD83 antibody APC-65171Download protocol
Standard Protocols
Click here to view our Standard Protocols