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验证数据展示

  • FC experiment of Human Monocytes-derived dendritic cells using 65171-1-Ig

    FC experiment of Human Monocytes-derived dendritic cells using 65171-1-Ig

    1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 0.5 ug Anti-Human CD83 (65171-1-Ig, Clone:HB15e) and FITC anti-Mouse IgG1 Antibody at dilution 1:50 (green), or stained with 0.5 ug mouse IgG1 isotype control and FITC anti-Mouse IgG1 Antibody at dilution 1:50 (black). Cells were not fixed.

Anti-Human CD83 (HB15e)

CD83 Monoclonal Antibody for FC
Cat No. 65171-1-Ig

产品说明书

CloneNo. HB15e

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human, non-human primates

应用

FC

别名

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inHuman monocytes-derived dendritic cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65171-1-Ig targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

经测试应用 FC Application Description
经测试反应性 human, non-human primates
免疫原 Human CD83-transfected Cos cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD83 molecule
别名 B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule
计算分子量 205 aa, 23 kDa
GenBank蛋白编号BC030830
基因名称 CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
RRIDAB_2918447
偶联类型 Unconjugated
形式 Liquid
纯化方式Purified by protein-A affinity chromatography
UNIPROT IDQ01151
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

实验方案

Product Specific Protocols
FC protocol for CD83 antibody 65171-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols