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验证数据展示

  • FC experiment of human PBMCs using CL594-65610

    FC experiment of human PBMCs using CL594-65610

    1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human CD79a (HM57) Mouse IgG2a RecAb (CL594-65610, Clone: HM57) or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4). Cells were co-stained with CoraLite® Plus 488 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using CL594-65610

    FC experiment of human PBMCs using CL594-65610

    1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human CD79a (HM57) Mouse IgG2a RecAb (CL594-65610, Clone: HM57) (red) or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594 Anti-Human CD79a (HM57) Mouse IgG2a Recombinant Antibody

CD79a Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. CL594-65610

产品说明书

CloneNo. HM57

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

B-cell antigen receptor complex-associated protein alpha chain, MB 1, MB 1 membrane glycoprotein, MB1, MB-1 membrane glycoprotein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD79a Ready-To-Use IHC Kit. CD79a primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65610-1-MR

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65610 targets CD79a in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD79a molecule, immunoglobulin-associated alpha
别名 B-cell antigen receptor complex-associated protein alpha chain, MB 1, MB 1 membrane glycoprotein, MB1, MB-1 membrane glycoprotein
GenBank蛋白编号BC113731
基因名称 CD79A
Gene ID (NCBI) 973
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11912
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79a is expressed in B cell and localizes in the cell membrane. CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79a is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

实验方案

Product Specific Protocols
FC protocol for CL594 CD79a antibody CL594-65610Download protocol
Standard Protocols
Click here to view our Standard Protocols