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CD79A Polyclonal antibody

CD79A Polyclonal Antibody for FC, IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22349-1-AP

Print datasheet

Synonyms

CD79A, Ig alpha, IGA, MB 1, MB 1 membrane glycoprotein, MB1, Surface IgM associated protein

验证数据展示

  • WB analysis using 22349-1-AP

    WB analysis using 22349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22349-1-AP (CD79A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 22349-1-AP

    IHC staining of human tonsillitis using 22349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD79A antibody included
  • IHC staining of human tonsillitis using 22349-1-AP

    IHC staining of human tonsillitis using 22349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD79A antibody included
  • IF Staining of human tonsillitis using 22349-1-AP

    IF Staining of human tonsillitis using 22349-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD79A antibody (22349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human tonsillitis using 22349-1-AP

    IF Staining of human tonsillitis using 22349-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD79A antibody (22349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Raji using 22349-1-AP

    FC experiment of Raji using 22349-1-AP

    1X10^6 Raji cells were stained with 0.20 ug Anti-Human CD79A (22349-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy CD79A Ready-To-Use IHC Kit. CD79A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22349-1-AP is CD79A Fusion Protein expressed in E. coli.

Tested Applications FC, IF-P, IHC, WB, ELISA
Tested Reactivity human
Immunogen CD79A fusion protein Ag17924 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD79a molecule, immunoglobulin-associated alpha
Synonyms CD79A, Ig alpha, IGA, MB 1, MB 1 membrane glycoprotein, MB1, Surface IgM associated protein
Calculated Molecular Weight 226 aa, 25 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC113733
Gene Symbol CD79A
Gene ID (NCBI) 973
RRIDAB_2879084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11912
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD79A, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

实验方案

Product Specific Protocols
WB protocol for CD79A antibody 22349-1-APDownload protocol
IHC protocol for CD79A antibody 22349-1-APDownload protocol
IF protocol for CD79A antibody 22349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols