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验证数据展示

  • IHC staining of mouse spleen using 85802-2-RR

    IHC staining of mouse spleen using 85802-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85802-2-RR (CD74 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 85802-2-RR

    IHC staining of mouse spleen using 85802-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85802-2-RR (CD74 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD74 Recombinant antibody

CD74 Uni-rAbTM Recombinant Antibody for IHC, ELISA
Cat No. 85802-2-RR

产品说明书

CloneNo. 250109B11

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, ELISA

别名

Class-II-associated invariant chain peptide, CLIP, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85802-2-RR targets CD74 in IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 mouse
免疫原 CD74 fusion protein Eg2716 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
别名 Class-II-associated invariant chain peptide, CLIP, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii
计算分子量32 kDa
GenBank蛋白编号NM_001042605.1
基因名称 Cd74
Gene ID (NCBI) 16149
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04441-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165; PMID: 36712241).

实验方案

Product Specific Protocols
IHC protocol for CD74 antibody 85802-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols