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验证数据展示

  • FC experiment of human PBMCs using Biotin-65564

    FC experiment of human PBMCs using Biotin-65564

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD73 (AD2) Mouse IgG2a RecAb (Biotin-65564, Clone: AD2) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with APC Anti-Human CD45. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using Biotin-65564

    FC experiment of human PBMCs using Biotin-65564

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD73 (AD2) Mouse IgG2a RecAb (Biotin-65564, Clone: AD2) (red) and CoraLite®488-conjugated streptavidin, or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Biotin Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Antibody

CD73 Uni-rAbTM Recombinant Antibody for FC
Cat No. Biotin-65564

产品说明书

CloneNo. AD2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65564 targets CD73 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Pre-B leukemia cell line 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 5'-nucleotidase, ecto (CD73)
别名 NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase
计算分子量 29 kDa, 63 kDa
GenBank蛋白编号BC015940
基因名称 CD73
Gene ID (NCBI) 4907
偶联类型 Biotin
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21589
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
FC protocol for Biotin CD73 antibody Biotin-65564Download protocol
Standard Protocols
Click here to view our Standard Protocols