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验证数据展示

  • WB analysis using 84766-4-RR

    WB analysis using 84766-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84766-4-RR (Transferrin R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 84766-4-RR

    IF Staining of RAW 264.7 using 84766-4-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD71 antibody (84766-4-RR, Clone: 242122B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84766-4-RR

    Affinity and Kinetic Characterization of 84766-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84766-4-RR against Mouse CD71 were performed. The affinity constant is 1.04 nM.

CD71 Recombinant antibody

CD71 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84766-4-RR

产品说明书

CloneNo. 242122B2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

Tfrc, transferrin receptor, TR, TFR1, TFR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse placenta tissue
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84766-4-RR targets CD71 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transferrin receptor
别名 Tfrc, transferrin receptor, TR, TFR1, TFR
计算分子量86kDa
观测分子量85-100 kDa
GenBank蛋白编号NM_011638.4
基因名称 CD71
Gene ID (NCBI) 22042
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ62351
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

实验方案

Product Specific Protocols
WB protocol for CD71 antibody 84766-4-RRDownload protocol
IF protocol for CD71 antibody 84766-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols