• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • IF Staining of K-562 using 81793-3-RR

    IF Staining of K-562 using 81793-3-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-3-RR, Clone: 250738C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human placenta using 81793-3-RR

    IHC staining of human placenta using 81793-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD71 antibody included
  • IHC staining of human placenta using 81793-3-RR

    IHC staining of human placenta using 81793-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD71 antibody included
  • IHC staining of human tonsillitis using 81793-3-RR

    IHC staining of human tonsillitis using 81793-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD71 antibody included
  • Affinity of 81793-3-RR

    Affinity of 81793-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81793-3-RR against Human CD71 were performed. The affinity constant is 31.5 pM.

CD71 Recombinant monoclonal antibody

CD71 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 81793-3-RR

产品说明书

CloneNo. 250738C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

p90, sTfR, T9, TFR, TFR1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81793-3-RR targets CD71 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0339

Product name: Recombinant Human Transferrin R/CD71 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*HIS

Domain: 89-760 aa of

Sequence: CKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTSTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transferrin receptor (p90, CD71)
别名 p90, sTfR, T9, TFR, TFR1
计算分子量85 kDa
基因名称 CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02786
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

实验方案

Product Specific Protocols
IF protocol for CD71 antibody 81793-3-RRDownload protocol
IHC protocol for CD71 antibody 81793-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...