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验证数据展示

  • WB analysis of Raji using 66180-1-Ig (same clone as 66180-1-PBS)

    WB analysis of Raji using 66180-1-Ig (same clone as 66180-1-PBS)

    Untreated and PNGase F-treated lysates of Raji cells and HeLa cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • WB analysis using 66180-1-Ig (same clone as 66180-1-PBS)

    WB analysis using 66180-1-Ig (same clone as 66180-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

    WB analysis of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

    WB result of CD71 antibody (66180-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD71 transfected HeLa cells. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IF Staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    IF Staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66180-1-Ig (same clone as 66180-1-PBS)

    IF Staining of MCF-7 using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

    IP experiment of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

    IP result of anti-CD71 (IP:66180-1-Ig, 5ug; Detection:66180-1-Ig 1:1000) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IF Staining of K-562 using 66180-1-Ig (same clone as 66180-1-PBS)

    IF Staining of K-562 using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 66180-1-Ig (same clone as 66180-1-PBS)

    IF Staining of K-562 using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CD71 Monoclonal antibody, PBS Only

CD71 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 66180-1-PBS

产品说明书

CloneNo. 3C11F11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA

别名

TFR1, TFR, T9, sTfR, p90

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.

产品信息

66180-1-PBS targets CD71 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
经测试反应性 human
免疫原 CD71 fusion protein Ag21612 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transferrin receptor (p90, CD71)
别名 TFR1, TFR, T9, sTfR, p90
计算分子量 85 kDa
观测分子量 90 kDa
GenBank蛋白编号BC001188
基因名称 CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
RRIDAB_2881575
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02786
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.