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验证数据展示

  • WB analysis using 30574-1-AP (same clone as 30574-1-PBS)

    WB analysis using 30574-1-AP (same clone as 30574-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30574-1-AP (CD71 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 30574-1-AP (same clone as 30574-1-PBS)

    IHC staining of human placenta using 30574-1-AP (same clone as 30574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30574-1-AP (CD71 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IHC staining of human placenta using 30574-1-AP (same clone as 30574-1-PBS)

    IHC staining of human placenta using 30574-1-AP (same clone as 30574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30574-1-AP (CD71 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IP experiment of HeLa using 30574-1-AP (same clone as 30574-1-PBS)

    IP experiment of HeLa using 30574-1-AP (same clone as 30574-1-PBS)

    IP result of anti-CD71 (IP:30574-1-AP, 4ug; Detection:30574-1-AP 1:10000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 30574-1-AP (same clone as 30574-1-PBS)

    IF Staining of HeLa using 30574-1-AP (same clone as 30574-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD71 antibody (30574-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 30574-1-AP (same clone as 30574-1-PBS)

    IF Staining of MCF-7 using 30574-1-AP (same clone as 30574-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (30574-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

CD71 Polyclonal antibody, PBS Only

CD71 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 30574-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

p90, sTfR, T9, TFR, TFR1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.

产品信息

30574-1-PBS targets CD71 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0339

Product name: Recombinant Human Transferrin R/CD71 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*HIS

Domain: 89-760 aa of

Sequence: CKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTSTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transferrin receptor (p90, CD71)
别名 p90, sTfR, T9, TFR, TFR1
计算分子量85 kDa
观测分子量80-90 kDa
基因名称 CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02786
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.