• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68827-1-Ig

    WB analysis using 68827-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68827-1-Ig (CD69 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CD69 Monoclonal antibody

CD69 Monoclonal Antibody for WB, ELISA
Cat No. 68827-1-Ig

产品说明书

CloneNo. 3D10A2

宿主/亚型

Mouse / IgG3

种属反应性

Human

应用

WB, ELISA

别名

Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inU-937 cells, THP-1 cells, HL-60 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68827-1-Ig targets CD69 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 CD69 fusion protein Ag30963 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 CD69 molecule
别名 Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3
计算分子量 23 kDa
观测分子量30 kDa
GenBank蛋白编号BC007037
基因名称 CD69
Gene ID (NCBI) 969
RRIDAB_3670438
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07108
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, and several subsets of tissue-resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in regulating immune responses (PMID: 15745855).

实验方案

Product Specific Protocols
WB protocol for CD69 antibody 68827-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols