CD69 Polyclonal antibody

CD69 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10803-1-AP

Print datasheet

Synonyms

Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3



经过测试的应用

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10803-1-AP targets CD69 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB, IF, FC, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CD69 fusion protein Ag1094 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD69 molecule
Synonyms Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 28 kDa, 32 kDa
GenBank Accession NumberBC007037
Gene Symbol CD69
Gene ID (NCBI) 969
RRIDAB_2074965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07108
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.

实验方案

Product Specific Protocols
WB protocol for CD69 antibody 10803-1-APDownload protocol
IHC protocol for CD69 antibody 10803-1-APDownload protocol
IF protocol for CD69 antibody 10803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Expert Rev Clin Immunol

Juxtaposition of IL-1β and IFN-γ expression and apoptosis of keratinocytes in adult-onset Still's disease.

Authors - Shijia Rao
IF

Cell Rep

Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.

Authors - Huiwen Zheng
humanIHC

Oncoimmunology

Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.

Authors - Chen Zhu
mouseFC

J Nanobiotechnology

Optogenetic engineered umbilical cord MSC-derived exosomes for remodeling of the immune microenvironment in diabetic wounds and the promotion of tissue repair

Authors - Xin Zhao
humanWB

Aging (Albany NY)

An integrated bioinformatic investigation of focal adhesion-related genes in glioma followed by preliminary validation of COL1A2 in tumorigenesis

Authors - Guojun Yao