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Species Reactivity
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验证数据展示

  • FC experiment of human PBMCs using 65202-1-Ig

    FC experiment of human PBMCs using 65202-1-Ig

    1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65202-1-Ig, Clone:KP1) and FITC-Donkey anti-Mouse IgG at dilution 1:1000 (red), or Mouse IgG1 Isotype Control and FITC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using 65202-1-Ig

    FC experiment of human PBMCs using 65202-1-Ig

    1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65202-1-Ig, Clone:KP1) and FITC-Donkey anti-Mouse IgG at dilution 1:1000, or Mouse IgG1 Isotype Control and FITC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

Anti-Human CD68 (KP1)

CD68 Monoclonal Antibody for FC (Intra)
Cat No. 65202-1-Ig

产品说明书

CloneNo. KP1

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC (Intra)

别名

CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension
Flow Cytometry (FC)FC :
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65202-1-Ig targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 Lysosomal fraction of human alveolar macrophages 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD68 molecule
别名 CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1
计算分子量 37 kDa
GenBank蛋白编号BC015557
基因名称 CD68
Gene ID (NCBI) 968
RRIDAB_3084903
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP34810
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for CD68 antibody 65202-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols