验证数据展示
经过测试的应用
| Positive IF/ICC detected in | THP-1 cells |
| Positive FC (Intra) detected in | human PBMCs |
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65187-1-Ig targets CD68 in WB, IHC, IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Lysosomal contents of lung macrophages 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD68 molecule |
| 别名 | Y1/82A, SCARD1, Macrosialin, GP110, CD68 molecule |
| 计算分子量 | 37 kDa |
| GenBank蛋白编号 | BC015557 |
| 基因名称 | CD68 |
| Gene ID (NCBI) | 968 |
| RRID | AB_2918458 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P34810 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CD68 antibody 65187-1-Ig | Download protocol |
| FC protocol for CD68 antibody 65187-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Diosgenin alters LPS-induced macrophage polarization by activating PPARγ/NF-κB signaling pathway | ||
Phytomedicine Koumine regulates macrophage M1/M2 polarization via TSPO, alleviating sepsis-associated liver injury in mice | ||
Int Immunopharmacol Macrophages regulates the transition of pericyte to peritoneal fibrosis through the GSDMD/IL-1β axis | ||
Hepatology Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis | ||
Exp Neurol Microglia trigger the structural plasticity of GABAergic neurons in the hippocampal CA1 region of a lipopolysaccharide-induced neuroinflammation model | ||
Int Immunopharmacol Inhibition of aquaporin-9 ameliorates severe acute pancreatitis and associated lung injury by NLRP3 and Nrf2/HO-1 pathways |