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验证数据展示

  • WB analysis using 84518-3-RR

    WB analysis using 84518-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84518-3-RR (CD64 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 84518-3-RR

    IHC staining of mouse spleen using 84518-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84518-3-RR (CD64 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of RAW 264.7 using 84518-3-RR

    IF Staining of RAW 264.7 using 84518-3-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD64/FCGR1 antibody (84518-3-RR, Clone: 241809G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD64 Recombinant monoclonal antibody

CD64 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84518-3-RR

产品说明书

CloneNo. 241809G4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, ELISA

别名

241809G4, Fcg1, Fc-gamma RI, Fcgr1, FcRI

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells, J774A.1 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84518-3-RR targets CD64 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2107

Product name: Recombinant Mouse CD64 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 25-297 aa of NM_010186.5

Sequence: EVVNATKAVITLQPPWVSIFQKENVTLWCEGPHLPGDSSTQWFINGTAVQISTPSYSIPEASFQDSGEYRCQIGSSMPSDPVQLQIHNDWLLLQASRRVLTEGEPLALRCHGWKNKLVYNVVFYRNGKSFQFSSDSEVAILKTNLSHSGIYHCSGTGRHRYTSAGVSITVKELFTTPVLRASVSSPFPEGSLVTLNCETNLLLQRPGLQLHFSFYVGSKILEYRNTSSEYHIARAEREDAGFYWCEVATEDSSVLKRSPELELQVLGPQSSAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc receptor, IgG, high affinity I
别名 241809G4, Fcg1, Fc-gamma RI, Fcgr1, FcRI
计算分子量45 kDa
观测分子量68-72 kDa
GenBank蛋白编号NM_010186.5
基因名称 Fcgr1
Gene ID (NCBI) 14129
RRIDAB_3672025
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26151
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

实验方案

Product Specific Protocols
IF protocol for CD64 antibody 84518-3-RRDownload protocol
IHC protocol for CD64 antibody 84518-3-RRDownload protocol
WB protocol for CD64 antibody 84518-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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