CD63 Monoclonal antibody
CD63 Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3D4D1
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U2OS cells, MCF-7 cells, A549 cells, K-562 cells, HL-60, THP-1 cells, LNCaP cells, HUVEC cells |
| Positive IHC detected in | human tonsillitis tissue, human malignant melanoma tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue, human malignant melanoma tissue, human lymphoma tissue |
| Positive FC detected in | THP-1 cells |
Planning an IHC experiment? We recommend our IHCeasy CD63 Ready-To-Use IHC Kit. CD63 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:350-1:1400 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67605-1-Ig targets CD63 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | FC, IF-P, IHC, WB, ELISA |
| Cited Applications | WB, FC |
| Tested Reactivity | Human |
| Cited Reactivity | human, rat, goat |
| Immunogen | CD63 fusion protein Ag19690 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD63 molecule |
| Synonyms | CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC002349 |
| Gene Symbol | CD63 |
| Gene ID (NCBI) | 967 |
| RRID | AB_2882811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08962 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD63 antibody 67605-1-Ig | Download protocol |
| IHC protocol for CD63 antibody 67605-1-Ig | Download protocol |
| IF protocol for CD63 antibody 67605-1-Ig | Download protocol |
| FC protocol for CD63 antibody 67605-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Drug Des Devel Ther Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling | ||
Oxid Med Cell Longev Adipose-Derived Circulating Exosomes Promote Protection of the Pulmonary Endothelial Barrier by Inhibiting EndMT and Oxidative Stress through Down-Regulation of the TGF-β Pathway: A Potential Explanation for the Obesity Paradox in ARDS. | ||
Aging (Albany NY) MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat. | ||
Aging (Albany NY) Exosomal circRNAs contribute to intestinal development via the VEGF signalling pathway in human term and preterm colostrum. | ||
Aging (Albany NY) Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway. | ||
Int J Mol Sci MicroRNAs in Small Extracellular Vesicles from Amniotic Fluid and Maternal Plasma Associated with Fetal Palate Development in Mice |
