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APC Anti-Human CD61 (VIPL2)

CD61 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

VIPL2

Cat No : APC-65185

Print datasheet

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

验证数据展示

  • FC experiment of human peripheral blood platelets using APC-65185

    FC experiment of human peripheral blood platelets using APC-65185

    1X10^6 human peripheral blood platelets were surface stained with 10.00 ul/test APC Anti-Human CD61 (APC-65185, Clone:VIPL2) (red) or isotype control antibody (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood platelets
Planning an IHC experiment? We recommend our IHCeasy ITGB3/CD61 Ready-To-Use IHC Kit. ITGB3/CD61 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 μl per 10^6 cells in a 100 µl suspension or 10 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

APC-65185 targets CD61 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Applications FC
Cited ApplicationsFC
Tested Reactivity Human, Non-human primates
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Synonyms CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3
Calculated Molecular Weight 788 aa, 87 kDa
GenBank Accession NumberBC127666
Gene Symbol ITGB3
Gene ID (NCBI) 3690
RRIDAB_2883045
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP05106
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD61 is a 110-kDa membrane glycoprotein that is also termed integrin β3 chain or GPIIIa. CD61 can associate with integrin αV chain (CD51) or integrin αIIb chain (CD41, GPIIb) to form heterodimeric receptors for cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, plasminogen, prothrombin, thrombospondin, vitronectin and von Willebrand factor (PMID: 7694604; 9251239; 2018971; 20628538). Integrin αVβ3 (CD51-CD61) is present on endothelium, smooth muscle, osteoclasts, platelets, some fibroblasts, some activated leukocytes and tumor cells (PMID: 7694604). GPllb-llla (CD41-CD61) is restricted in distribution to platelets and cells of megakaryoblastic potential and is required for platelet aggregation (PMID: 2018971; 16322781).

实验方案

Product Specific Protocols
FC protocol for APC CD61 antibody APC-65185Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
FC

J Ovarian Res

Novel prognostic marker TGFBI affects the migration and invasion function of ovarian cancer cells and activates the integrin αvβ3-PI3K-Akt signaling pathway

Authors - Hao Wang
View Article