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验证数据展示

  • WB analysis of human plasma using 17224-1-AP (same clone as 17224-1-PBS)

    WB analysis of human plasma using 17224-1-AP (same clone as 17224-1-PBS)

    human plasma was subjected to SDS PAGE followed by western blot with 17224-1-AP (CD5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 17224-1-AP (same clone as 17224-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 17224-1-AP (same clone as 17224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17224-1-AP (CD5L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 17224-1-AP (same clone as 17224-1-PBS)

    IF Staining of HepG2 using 17224-1-AP (same clone as 17224-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD5L antibody (17224-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

CD5L Polyclonal antibody, PBS Only

CD5L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17224-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

hAIM, CT-2, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, API6

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17224-1-PBS targets CD5L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11062

Product name: Recombinant human CD5L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-347 aa of BC033586

Sequence: SPSGVRLVGGLHRCEGRVEVEQKGQWGTVCDDGWDIKDVAVLCRELGCGAASGTPSGILYEPPAEKEQKVLIQSVSCTGTEDTLAQCEQEEVYDCSHDEDAGASCENPESSFSPVPEGVRLADGPGHCKGRVEVKHQNQWYTVCQTGWSLRAAKVVCRQLGCGRAVLTQKRCNKHAYGRKPIWLSQMSCSGREATLQDCPSGPWGKNTCNHDEDTWVECEDPFDLRLVGGDNLCSGRLEVLHKGVWGSVCDDNWGEKEDQVVCKQLGCGKSLSPSFRDRKCYGPGVGRIWLDNVRCSGEEQSLEQCQHRFWGFHDCTHQEDVAVICSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD5 molecule-like
别名 hAIM, CT-2, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, API6
计算分子量 347 aa, 38 kDa
观测分子量40 kDa
GenBank蛋白编号BC033586
基因名称 CD5L
Gene ID (NCBI) 922
RRIDAB_3085517
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43866
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).

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