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- KD/KO Validated
CD59 Polyclonal antibody
CD59 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, human brain tissue, HUVEC cells, HeLa cells |
| Positive IHC detected in | human placenta tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | BxPC-3 cells |
| Positive FC detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10742-1-AP targets CD59 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | CD59 fusion protein Ag1173 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD59 molecule, complement regulatory protein |
| Synonyms | 16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | BC001506 |
| Gene Symbol | CD59 |
| Gene ID (NCBI) | 966 |
| RRID | AB_2076228 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13987 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD59 antibody 10742-1-AP | Download protocol |
| IHC protocol for CD59 antibody 10742-1-AP | Download protocol |
| IF protocol for CD59 antibody 10742-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium | ||
Int J Oncol The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer. | ||
Chem Res Toxicol 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells. | ||
Biochem Biophys Rep Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica. | ||
Mol Cell Endocrinol Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.
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