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验证数据展示

  • WB analysis using 32061-1-AP

    WB analysis using 32061-1-AP

    Mouse spleen tissue was subjected to SDS PAGE followed by western blot with 32061-1-AP (CD55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 32061-1-AP

    IHC staining of mouse lung using 32061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32061-1-AP (CD55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 32061-1-AP

    IHC staining of mouse spleen using 32061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32061-1-AP (CD55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 32061-1-AP

    IHC staining of mouse spleen using 32061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32061-1-AP (CD55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD55 Polyclonal antibody

CD55 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32061-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

DAF-GPI, Daf1, Daf, Complement decay-accelerating factor, GPI-anchored, Cd55a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32061-1-AP targets CD55 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD55 antigen
别名 DAF-GPI, Daf1, Daf, Complement decay-accelerating factor, GPI-anchored, Cd55a
计算分子量43kDa
观测分子量60-70 kDa
GenBank蛋白编号NM_010016.3
基因名称 CD55
Gene ID (NCBI) 13136
RRIDAB_3670182
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ61475
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).

实验方案

Product Specific Protocols
WB protocol for CD55 antibody 32061-1-APDownload protocol
IHC protocol for CD55 antibody 32061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols