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验证数据展示

  • FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50085-1

    Sandwich ELISA standard curve of MP50085-1

    Sandwich ELISA standard curve of MP50085-1, ICAM-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65075-1-PBS. Detection antibody: 68693-1-PBS. Standard: Eg0570. Range: 62.5-4000 pg/mL.

  • Sample test of MP50085-1

    Sample test of MP50085-1

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean ICAM-1/CD54 concentration was determined to be 2,139.3 pg/mL in unstimulated PBMC supernatant, 30,678.8 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP50085-1

    Sample test of MP50085-1

    Saliva of seven individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 5,597.4 pg/mL with a range of 2,577.6 - 11,698.1 pg/mL.

  • Sample test of MP50085-1

    Sample test of MP50085-1

    Urine of seven individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 1,031.9 pg/mL with a range of 49.7 - 3,299.7 pg/mL.

  • Sample test of MP50085-1

    Sample test of MP50085-1

    Serum of sixteen individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 260.08 ng/mL with a range of 142.73 - 650.42 ng/mL.

Anti-Human ICAM-1/CD54 (15.2), PBS Only (Capture)

ICAM-1/CD54 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 65075-1-PBS

产品说明书

CloneNo. 15.2

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD54 (ICAM-1), CD54, ICAM-1, 15.2, BB2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

产品信息

65075-1-PBS targets ICAM-1/CD54 as part of a matched antibody pair:

MP50085-1: 65075-1-PBS capture and 68693-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Rheumatoid synovial cells and human monocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 intercellular adhesion molecule 1
别名 CD54 (ICAM-1), CD54, ICAM-1, 15.2, BB2
计算分子量 90 kDa
GenBank蛋白编号BC015969
基因名称 ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
RRIDAB_2918379
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP05362
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).