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验证数据展示

  • WB analysis of RAW 264.7 using 85838-3-RR (same clone as 85838-3-PBS)

    WB analysis of RAW 264.7 using 85838-3-RR (same clone as 85838-3-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85838-3-RR (CD53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85838-3-RR (same clone as 85838-3-PBS)

    IHC staining of mouse spleen using 85838-3-RR (same clone as 85838-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85838-3-RR (Cd53 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85838-3-RR (same clone as 85838-3-PBS)

    IHC staining of mouse spleen using 85838-3-RR (same clone as 85838-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85838-3-RR (Cd53 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

  • IF Staining of A20 using 85838-3-RR (same clone as 85838-3-PBS)

    IF Staining of A20 using 85838-3-RR (same clone as 85838-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A20 cells using Cd53 antibody (85838-3-RR, Clone: 250105D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

Mouse CD53 Recombinant monoclonal antibody, PBS Only

CD53 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85838-3-PBS

产品说明书

CloneNo. 250105D10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Cell surface glycoprotein CD53, Leukocyte surface antigen CD53

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85838-3-PBS targets CD53 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2684

Product name: Recombinant Mouse CD53 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 107-181 aa of NM_007651.3

Sequence: EQKLNTLVAEGLNDSIQHYHSDNSTMKAWDFIQTQLQCCGVNGSSDWTSGPPSSCPSGADVQGCYNKAKSWFHSN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD53 antigen
别名 Cell surface glycoprotein CD53, Leukocyte surface antigen CD53
计算分子量24 kDa
观测分子量35-40 kDa
GenBank蛋白编号NM_007651.3
基因名称 Cd53
Gene ID (NCBI) 12508
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ61451
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).

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