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验证数据展示

  • WB analysis using 84837-1-RR

    WB analysis using 84837-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84837-1-RR (CD53 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of Daudi using 84837-1-RR

    IF Staining of Daudi using 84837-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of Jurkat using 84837-1-RR

    IF Staining of Jurkat using 84837-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human tonsillitis using 84837-1-RR

    IHC staining of human tonsillitis using 84837-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84837-1-RR (CD53 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84837-1-RR

    IHC staining of human tonsillitis using 84837-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84837-1-RR (CD53 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84837-1-RR

    Affinity and Kinetic Characterization of 84837-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84837-1-RR against Human CD53 were performed. The affinity constant is below 1 pM.

CD53 Recombinant antibody

CD53 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84837-1-RR

产品说明书

CloneNo. 242038C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

242038C5, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin 25

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Ramos cells, IM-9 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells, Daudi cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84837-1-RR targets CD53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CD53 fusion protein Eg2182 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD53 molecule
别名 242038C5, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin 25
计算分子量24kDa
观测分子量 40 kDa
GenBank蛋白编号NM_000560.4
基因名称 CD53
Gene ID (NCBI) 963
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP19397
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).

实验方案

Product Specific Protocols
WB protocol for CD53 antibody 84837-1-RRDownload protocol
IF protocol for CD53 antibody 84837-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols