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验证数据展示

  • WB analysis of EL-4 using 83451-4-RR

    WB analysis of EL-4 using 83451-4-RR

    EL-4 cells were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 83451-4-RR

    IHC staining of mouse spleen using 83451-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83451-4-RR

    IHC staining of mouse spleen using 83451-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 83451-4-RR

    IF Staining of mouse spleen using 83451-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD5 antibody (83451-4-RR, Clone: 240244C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83451-4-RR

    Affinity and Kinetic Characterization of 83451-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83451-4-RR against Mouse CD5 were performed. The affinity constant is 89.9 pM.

CD5 Recombinant monoclonal antibody

CD5 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83451-4-RR

产品说明书

CloneNo. 240244C2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, ELISA

别名

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEL-4 cells, mouse thymus tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83451-4-RR targets CD5 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD5 antigen
别名 Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen
计算分子量54 kDa
观测分子量 55 kDa, 67 kDa
GenBank蛋白编号NM_007650.3
基因名称 Cd5
Gene ID (NCBI) 12507
RRIDAB_3671088
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP13379
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
IF protocol for CD5 antibody 83451-4-RRDownload protocol
IHC protocol for CD5 antibody 83451-4-RRDownload protocol
WB protocol for CD5 antibody 83451-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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