验证数据展示
经过测试的应用
| Positive WB detected in | EL-4 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31249-1-AP targets CD5 in WB, ELISA applications and shows reactivity with Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD5 antigen |
| 别名 | Cd5, CD5 antigen, Ly 1, Ly 12, Ly A, Lyt 1 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | NM_007650.3 |
| 基因名称 | Cd5 |
| Gene ID (NCBI) | 12507 |
| RRID | AB_3669918 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13379 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD5 antibody 31249-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |