验证数据展示
经过测试的应用
Positive WB detected in | CTLL-2 cells, EL-4 cells, mouse spleen tissue |
Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31774-1-AP targets CD48 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | mouse |
免疫原 | CD48 fusion protein Eg1323 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | CD48 antigen |
别名 | BLAST, BLAST 1, BLAST1, CD48 antigen, MEM 102 |
计算分子量 | 27 kDa |
观测分子量 | 45-55 kDa |
GenBank蛋白编号 | BAE96326.1 |
基因名称 | Cd48 |
Gene ID (NCBI) | 12506 |
RRID | AB_3670109 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | P18181 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.
实验方案
Product Specific Protocols | |
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WB protocol for CD48 antibody 31774-1-AP | Download protocol |
IHC protocol for CD48 antibody 31774-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |