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验证数据展示

  • WB analysis using 31774-1-AP

    WB analysis using 31774-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31774-1-AP (CD48/SLAMF2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 31774-1-AP

    IHC staining of mouse spleen using 31774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31774-1-AP (CD48 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31774-1-AP

    IHC staining of mouse spleen using 31774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31774-1-AP (CD48 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD48 Polyclonal antibody

CD48 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31774-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

BLAST, BLAST 1, BLAST1, CD48 antigen, MEM 102

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCTLL-2 cells, EL-4 cells, mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31774-1-AP targets CD48 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 CD48 fusion protein Eg1323 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD48 antigen
别名 BLAST, BLAST 1, BLAST1, CD48 antigen, MEM 102
计算分子量27 kDa
观测分子量45-55 kDa
GenBank蛋白编号BAE96326.1
基因名称 Cd48
Gene ID (NCBI) 12506
RRIDAB_3670109
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP18181
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

实验方案

Product Specific Protocols
WB protocol for CD48 antibody 31774-1-APDownload protocol
IHC protocol for CD48 antibody 31774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols