CoraLite® Plus 488-conjugated CD47 Polyclonal antibody
CD47 Polyclonal Antibody for FC
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | HUVEC cells |
For other applications, we recommend the unconjugated version of this antibody, 29555-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-29555 targets CD47 in FC applications and shows reactivity with Human samples.
Tested Applications | FC |
Tested Reactivity | Human |
Immunogen | fusion protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD47 molecule |
Synonyms | CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6 |
Calculated Molecular Weight | 323 aa, 35 kDa |
GenBank Accession Number | BC010016 |
Gene Symbol | CD47 |
Gene ID (NCBI) | 961 |
RRID | AB_2919215 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q08722 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD47 antibody CL488-29555 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |