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CoraLite® Plus 488-conjugated CD47 Polyclonal antibody

CD47 Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-29555

Print datasheet

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

验证数据展示

  • FC experiment of HUVEC using CL488-29555

    FC experiment of HUVEC using CL488-29555

    1X10^6 HUVEC cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Human CD47 (CL488-29555) (red), or 0.25 ug Control Antibody. Cells were not fixed.

经过测试的应用

Positive FC detected inHUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 29555-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29555 targets CD47 in FC applications and shows reactivity with Human samples.

Tested Applications FC
Tested Reactivity Human
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD47 molecule
Synonyms CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6
Calculated Molecular Weight 323 aa, 35 kDa
GenBank Accession NumberBC010016
Gene Symbol CD47
Gene ID (NCBI) 961
RRIDAB_2919215
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08722
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD47 antibody CL488-29555Download protocol
Standard Protocols
Click here to view our Standard Protocols