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  • Featured Product
  • KD/KO Validated

CD47 Monoclonal antibody, PBS Only

CD47 Monoclonal Antibody for WB,Indirect ELISA,FC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,Indirect ELISA,FC

Conjugate

Unconjugated

CloneNo.

1E1D8

Cat No : 66304-1-PBS

Print datasheet

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

验证数据展示

  • WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

    WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66304-1-Ig (same clone as 66304-1-PBS)

    WB analysis of A549 using 66304-1-Ig (same clone as 66304-1-PBS)

    WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

  • FC experiment of HUVEC using 66304-1-Ig (same clone as 66304-1-PBS)

    FC experiment of HUVEC using 66304-1-Ig (same clone as 66304-1-PBS)

    1X10^6 HUVEC cells were stained with 0.20 ug Anti-Human CD47 (66304-1-Ig, Clone:1E1D8) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

  • WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

    WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

产品信息

66304-1-PBS targets CD47 in WB, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,FC
Tested Reactivity human
Immunogen CD47 fusion protein Ag14132 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD47 molecule
Synonyms CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6
Calculated Molecular Weight 323 aa, 35 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC010016
Gene Symbol CD47
Gene ID (NCBI) 961
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08722
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.