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验证数据展示

  • IF Staining of A431 using 60551-1-Ig

    IF Staining of A431 using 60551-1-Ig

    Immunofluorescent analysis of un-fixed A431 cells using CD47 antibody (60551-1-Ig, Clone: 1A4C4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • IF Staining of A431 using 60551-1-Ig

    IF Staining of A431 using 60551-1-Ig

    Immunofluorescent analysis of un-fixed A431 cells using CD47 antibody (60551-1-Ig, Clone: 1A4C4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • FC experiment of human PBMCs using 60551-1-Ig

    FC experiment of human PBMCs using 60551-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 μg CD47 Monoclonal Antibody (60551-1-Ig, Clone: 1A4C4, right) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, left) was parallel stained as control. Cells were not fixed.

  • IF Staining of Jurkat using 60551-1-Ig

    IF Staining of Jurkat using 60551-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD47 antibody (60551-1-Ig, Clone: 1A4C4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

CD47 Monoclonal antibody

CD47 Monoclonal Antibody for IF/ICC, FC, ELISA
Cat No. 60551-1-Ig

产品说明书

CloneNo. 1A4C4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC, ELISA

别名

1A4C4, CD47 molecule, IAP, Integrin associated protein, Integrin-associated protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, A431 cells, Jurkat cells
Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60551-1-Ig targets CD47 in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg31499

Product name: Recombinant Human CD47 protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 19-139 aa of BC010016

Sequence: QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVCVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD47 molecule
别名 1A4C4, CD47 molecule, IAP, Integrin associated protein, Integrin-associated protein
计算分子量 323 aa, 35 kDa
GenBank蛋白编号BC010016
基因名称 CD47
Gene ID (NCBI) 961
RRIDAB_3670268
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ08722
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

实验方案

Product Specific Protocols
IF protocol for CD47 antibody 60551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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