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Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for FC, IF-P

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC, IF-P

Conjugate

Unconjugated

CloneNo.

UCHL1

Cat No : 65150-1-Ig

Print datasheet

Synonyms

B220, CD45R, CD45RO, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200, UCHL1

验证数据展示

  • FC experiment of human PBMCs using 65150-1-Ig

    FC experiment of human PBMCs using 65150-1-Ig

    1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD45RA and 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65150-1-Ig

    FC experiment of human PBMCs using 65150-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • IF Staining of human colon cancer using 65150-1-Ig

    IF Staining of human colon cancer using 65150-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CD45RO antibody (65150-1-Ig, Clone: UCHL1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IF detected inhuman colon cancer tissue
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65150-1-Ig targets CD45RO in IF, FC applications and shows reactivity with Human samples.

Tested Applications FC, IF-P
Tested Reactivity Human
Immunogen IL-2 dependent T cell line, CA1 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms B220, CD45R, CD45RO, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200, UCHL1
GenBank Accession NumberBC014239
Gene Symbol PTPRC
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2881476
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08575
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

实验方案

Product Specific Protocols
IF protocol for CD45RO antibody 65150-1-IgDownload protocol
FC protocol for CD45RO antibody 65150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols