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PE Anti-Mouse CD45.1 (A20)

CD45.1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

A20

Cat No : PE-65092

Print datasheet

Synonyms

B220, CD45.1, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

验证数据展示

  • FC experiment of mouse splenocytes using PE-65092

    FC experiment of mouse splenocytes using PE-65092

    1X10^6 SJL mouse splenocytes were surface stained with 0.20 ug PE-Anti-Mouse CD45.1 (PE-65092, clone A20) (red) or 0.20 ug PE-mouse IgG2a isotype control (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inSJL mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65092 targets CD45.1 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen SJL mouse thymocytes and splenocytes 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms B220, CD45.1, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200
GenBank Accession NumberBC028512
Gene Symbol Ptprc
Gene ID (NCBI) 19264
RRIDAB_2883886
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP06800
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

实验方案

Product Specific Protocols
FC protocol for PE CD45.1 antibody PE-65092Download protocol
Standard Protocols
Click here to view our Standard Protocols