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验证数据展示

  • WB analysis of rat spleen using 84369-5-RR

    WB analysis of rat spleen using 84369-5-RR

    rat spleen tissue was subjected to SDS PAGE followed by western blot with 84369-5-RR (CD44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 84369-5-RR

    IHC staining of rat spleen using 84369-5-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 84369-5-RR

    IHC staining of rat spleen using 84369-5-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NR8383 using 84369-5-RR

    IF Staining of NR8383 using 84369-5-RR

    Immunofluorescent analysis of (4% PFA) fixed NR8383 cells using CD44 antibody (84369-5-RR, Clone: 241648D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84369-5-RR

    Affinity and Kinetic Characterization of 84369-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84369-5-RR against Rat CD44 were performed. The affinity constant is 0.332 nM.

CD44 Recombinant antibody

CD44 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84369-5-RR

产品说明书

CloneNo. 241648D1

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, IF/ICC, ELISA

别名

Hermes antigen, GP90 lymphocyte homing/adhesion receptor, Extracellular matrix receptor III, ECMR-III, CD44 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat spleen tissue
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNR8383 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84369-5-RR targets CD44 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Cd44 molecule
别名 Hermes antigen, GP90 lymphocyte homing/adhesion receptor, Extracellular matrix receptor III, ECMR-III, CD44 antigen
计算分子量40 kDa
观测分子量80 kDa
GenBank蛋白编号XM_006234631.3
基因名称 Cd44
Gene ID (NCBI) 25406
RRIDAB_3671905
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO70509
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
WB protocol for CD44 antibody 84369-5-RRDownload protocol
IHC protocol for CD44 antibody 84369-5-RRDownload protocol
IF protocol for CD44 antibody 84369-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols