WB Figures
WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)
HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)
WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
WB analysis of human spleen using 60224-1-Ig (same clone as 60224-1-PBS)
human spleen tissue were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IF-P Figures
IF Staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)
Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
