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验证数据展示

  • WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

    WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

    IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 60224-1-Ig (same clone as 60224-1-PBS)

    IP experiment of HeLa using 60224-1-Ig (same clone as 60224-1-PBS)

    IP result of anti-CD44 (IP:60224-1-Ig, 4ug; Detection:60224-1-Ig 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

    IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD44 antibody included
  • IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD44 antibody included
  • WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)

    WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)

    WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD44 antibody included
  • IF Staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IF Staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IF Staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CD44 Monoclonal antibody, PBS Only

CD44 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 60224-1-PBS

产品说明书

CloneNo. 6F4H2

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA

别名

6F4H2, CD44 antigen, CDW44, CSPG8, ECMR III

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

产品信息

60224-1-PBS targets CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
经测试反应性 human, pig
免疫原 CD44 fusion protein Ag7633 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CD44 molecule (Indian blood group)
别名 6F4H2, CD44 antigen, CDW44, CSPG8, ECMR III
计算分子量 742 aa, 82 kDa
观测分子量 80-95 kDa
GenBank蛋白编号BC004372
基因名称 CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
RRIDAB_11042767
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16070
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.