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验证数据展示

  • WB analysis using 30854-1-AP

    WB analysis using 30854-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30854-1-AP (Cd44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 30854-1-AP

    IF Staining of RAW 264.7 using 30854-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD44 antibody (30854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30854-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Mouse, Rat and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

CD44, CD44 antigen, CDw44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, HERMES, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, Ly 24, MC56, MDU2, MDU3, MIC4, MUTCH I, Pgp 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJ774A.1 cells, RAW 264.7 cells, NR8383 cells
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

30854-1-AP targets CD44 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 Mouse, Rat
文献引用反应性rat
免疫原 CD44 fusion protein Ag33515 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD44 antigen
别名 CD44, CD44 antigen, CDw44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, HERMES, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, Ly 24, MC56, MDU2, MDU3, MIC4, MUTCH I, Pgp 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I
计算分子量86kd
观测分子量90 kDa
GenBank蛋白编号NM_009851
基因名称 Cd44
Gene ID (NCBI) 12505
RRIDAB_3086423
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP15379
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
WB protocol for CD44 antibody 30854-1-APDownload protocol
IF protocol for CD44 antibody 30854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Inflamm Regen

Macrophage depletion in inflamed rat knees prevents the activation of synovial mesenchymal stem cells by weakening Nampt and Spp1 signaling

Authors - Hayato Kodama
View Article