验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-66502 targets CD40L/CD154 in IHC applications and shows reactivity with human samples.
| 经测试应用 | IHC Application Description |
| 经测试反应性 | human |
| 免疫原 | CD40L/CD154 fusion protein Ag24941 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD40 ligand |
| 别名 | CD154, CD40L; CD154, CD40LG, CD40 ligand, CD40 L |
| 计算分子量 | 261 aa, 29 kDa |
| GenBank蛋白编号 | BC071754 |
| 基因名称 | CD40 Ligand |
| Gene ID (NCBI) | 959 |
| RRID | AB_2883075 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P29965 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kDa transmembrane homologue or 18-kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin CD40L/CD154 antibody Biotin-66502 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |