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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84406-2-RR (same clone as 84406-2-PBS)

    WB analysis using 84406-2-RR (same clone as 84406-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84406-2-RR (CD40/TNFRSF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84406-2-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 84406-2-RR (same clone as 84406-2-PBS)

    IF Staining of Raji using 84406-2-RR (same clone as 84406-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD40/TNFRSF5 antibody (84406-2-RR, Clone: 241760A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84406-2-PBS in a different storage buffer formulation.

CD40 Recombinant antibody, PBS Only

CD40 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84406-2-PBS

产品说明书

CloneNo. 241760A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

241760A11, B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84406-2-PBS targets CD40 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD40 fusion protein Eg1797 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD40 molecule, TNF receptor superfamily member 5
别名 241760A11, B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor
计算分子量31kDa
观测分子量45 kDa
GenBank蛋白编号NM_001250.6
基因名称 CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25942-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.