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验证数据展示

  • FC experiment of mouse splenocytes using AY7-65104

    FC experiment of mouse splenocytes using AY7-65104

    1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3, and 0.2 ug APC-Cyanine7 Anti-Mouse CD4 (GK1.5) (AY7-65104, Clone: GK1.5) or APC-Cyanine7 Rat IgG2b Isotype Control (LTF-2) (AY7-65211, Clone: LTF-2). Cells were not fixed.

  • FC experiment of mouse splenocytes using AY7-65104

    FC experiment of mouse splenocytes using AY7-65104

    1x10^6 mouse splenocytes were surface stained with 0.2 ug APC-Cyanine7 Anti-Mouse CD4 (GK1.5) (AY7-65104, Clone: GK1.5) (red) or APC-Cyanine7 Rat IgG2b Isotype Control (LTF-2) (AY7-65211, Clone: LTF-2) (blue). Cells were not fixed.

APC-Cyanine7 Anti-Mouse CD4 (GK1.5)

CD4 Monoclonal Antibody for FC
Cat No. AY7-65104

产品说明书

CloneNo. GK1.5

宿主/亚型

Rat / IgG2b, kappa

种属反应性

mouse

应用

FC

别名

CD4 antigen, GK1.5, L3T4, Ly 4, T-cell differentiation antigen L3T4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  APC-Cyanine7
规格价格库存

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经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.2 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

AY7-65104 targets CD4 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Mouse CTL clone V4 种属同源性预测
宿主/亚型 Rat / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD4 antigen
别名 CD4 antigen, GK1.5, L3T4, Ly 4, T-cell differentiation antigen L3T4
GenBank蛋白编号BC039137
基因名称 Cd4
Gene ID (NCBI) 12504
RRIDAB_3672360
偶联类型 APC-Cyanine7 Fluorescent Dye
最大激发/发射波长650 nm / 778 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP06332
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for APC-Cyanine7 CD4 antibody AY7-65104Download protocol
Standard Protocols
Click here to view our Standard Protocols