验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:450-1:1800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67786-1-Ig targets CD4 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC, IF, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag30870 Product name: Recombinant human CD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-226 aa of BC025782 Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD4 molecule |
| 别名 | 2H4A2, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 |
| 计算分子量 | 55 kDa |
| GenBank蛋白编号 | BC025782 |
| 基因名称 | CD4 |
| Gene ID (NCBI) | 920 |
| ENSEMBL Gene ID | ENSG00000010610 |
| RRID | AB_2918550 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P01730 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CD4 antibody 67786-1-Ig | Download protocol |
| IHC protocol for CD4 antibody 67786-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Sexual dimorphism of lung immune-regulatory units imprint biased pulmonary fibrosis | ||
Cancer Commun (Lond) Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies | ||
J Med Virol Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy | ||
Cardiovasc Res Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism | ||
Clin Transl Med Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy | ||
