CD4 Monoclonal antibody
CD4 Monoclonal Antibody for IF-P, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (4)
Applications
IF-P, IHC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
2H4A2
验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:450-1:1800 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67786-1-Ig targets CD4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IF-P, IHC, ELISA |
| Cited Applications | WB, IF, FC, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | CD4 fusion protein Ag30870 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD4 molecule |
| Synonyms | CD4, CD4 molecule, CD4mut |
| Calculated Molecular Weight | 55 kDa |
| GenBank Accession Number | BC025782 |
| Gene Symbol | CD4 |
| Gene ID (NCBI) | 920 |
| ENSEMBL Gene ID | ENSG00000010610 |
| RRID | AB_2918550 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01730 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD4 antibody 67786-1-Ig | Download protocol |
| IF protocol for CD4 antibody 67786-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Virol Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy | ||
Cardiovasc Res Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism | ||
Clin Transl Med Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy | ||
Front Immunol LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma | ||
Front Immunol Characteristics of the immunogenicity and tumor immune microenvironment in HER2-amplified lung adenocarcinoma | ||
Immunology Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis |
