• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 32406-1-AP

    WB analysis using 32406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32406-1-AP (CD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat thymus using 32406-1-AP

    IHC staining of rat thymus using 32406-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 32406-1-AP (CD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD4 Polyclonal antibody

CD4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32406-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, ELISA

别名

CD4 molecule, p55

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inrat spleen tissue, rat thymus tissue
Positive IHC detected inrat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32406-1-AP targets CD4 in WB, IHC, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 rat
免疫原 CD4 fusion protein Eg2025 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cd4 molecule
别名 CD4 molecule, p55
计算分子量51kDa
观测分子量 51-55 kDa
GenBank蛋白编号NM_012705.1
基因名称 Cd4
Gene ID (NCBI) 24932
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP05540
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

实验方案

Product Specific Protocols
WB protocol for CD4 antibody 32406-1-APDownload protocol
IHC protocol for CD4 antibody 32406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols