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CD3 Gamma Monoclonal antibody

CD3 Gamma Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

2E9G7

Cat No : 60347-1-Ig

Print datasheet

Synonyms

CD3 gamma, CD3G, T cell receptor T3 gamma chain, T3G

验证数据展示

  • WB analysis of Jurkat using 60347-1-Ig

    WB analysis of Jurkat using 60347-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60347-1-Ig (CD3 gamma antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60347-1-Ig

    IHC staining of human tonsillitis using 60347-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60347-1-Ig (CD3 gamma antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IHC staining of human tonsillitis using 60347-1-Ig

    IHC staining of human tonsillitis using 60347-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60347-1-Ig (CD3 gamma antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • FC experiment of Jurkat using 60347-1-Ig

    FC experiment of Jurkat using 60347-1-Ig

    1X10^6 Jurkat cells were stained with 0.2ug CD3 gamma antibody (60347-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

60347-1-Ig targets CD3 Gamma in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen CD3 Gamma fusion protein Ag15502 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD3g molecule, gamma (CD3-TCR complex)
Synonyms CD3 gamma, CD3G, T cell receptor T3 gamma chain, T3G
Calculated Molecular Weight 182 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC113830
Gene Symbol CD3G
Gene ID (NCBI) 917
RRIDAB_2881456
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09693
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

实验方案

Product Specific Protocols
WB protocol for CD3 Gamma antibody 60347-1-IgDownload protocol
IHC protocol for CD3 Gamma antibody 60347-1-IgDownload protocol
FC protocol for CD3 Gamma antibody 60347-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Sci

Metformin changes the immune microenvironment of colorectal cancer in patients with type 2 diabetes mellitus.

Authors - Akira Saito
View Article