CD3 Gamma Polyclonal antibody

CD3 Gamma Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21120-1-AP

Print datasheet

Synonyms

CD3 gamma, CD3G, CD3-gamma, T cell receptor T3 gamma chain, T3G



经过测试的应用

Positive WB detected inJurkat cells, mouse thymus tissue, MOLT-4 cells
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

21120-1-AP targets CD3 Gamma in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CD3 Gamma fusion protein Ag15475 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD3g molecule, gamma (CD3-TCR complex)
Synonyms CD3 gamma, CD3G, CD3-gamma, T cell receptor T3 gamma chain, T3G
Calculated Molecular Weight 182 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC113830
Gene Symbol CD3 Gamma
Gene ID (NCBI) 917
RRIDAB_10732688
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09693
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

实验方案

Product Specific Protocols
WB protocol for CD3 Gamma antibody 21120-1-APDownload protocol
IHC protocol for CD3 Gamma antibody 21120-1-APDownload protocol
IP protocol for CD3 Gamma antibody 21120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Mol Gastroenterol Hepatol

Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice.

Authors - Sohji Nishina

Life Sci Alliance

Sleep-wake patterns are altered with age, Prdm13 signaling in the DMH, and diet restriction in mice

Authors - Shogo Tsuji
mouseIHC

Front Oncol

Hyperosmotic cold shock mouse melanoma cells encapsulated with doxorubicin for targeted treatment of melanoma

Authors - Weihui Kong