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CD3 Monoclonal antibody

CD3 Monoclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC, IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3F3A1

Cat No : 60181-1-Ig

Print datasheet

Synonyms

CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE



经过测试的应用

Positive WB detected inMOLT-4 cells, Jurkat cells, human PBMCs
Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, Jurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3200-1:12800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60181-1-Ig targets CD3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsIF, FC, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen CD3 fusion protein Ag11797 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE
Calculated Molecular Weight 207 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3E
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
RRIDAB_10859262
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.

实验方案

Product Specific Protocols
WB protocol for CD3 antibody 60181-1-IgDownload protocol
IHC protocol for CD3 antibody 60181-1-IgDownload protocol
IF protocol for CD3 antibody 60181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC,IF

Cancer Commun (Lond)

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

Authors - Lu Tang
humanIF

J Med Virol

Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy

Authors - Xinyue Deng
humanIF

EBioMedicine

Patient-derived melanoma organoid models facilitate the assessment of immunotherapies

Authors - Lingling Ou
IHC

Autophagy

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.

Authors - Jingru Bai
humanIF

Sci Signal

ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis

Authors - Hao Cai
IHC

Carbohydr Polym

Multifunctional chitin-based hollow nerve conduit for peripheral nerve regeneration and neuroma inhibition.

Authors - Xiaqing Yang