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验证数据展示

  • WB analysis using 60181-1-Ig

    WB analysis using 60181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60181-1-Ig (CD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 60181-1-Ig

    WB analysis of Jurkat using 60181-1-Ig

    WB result of CD3 antibody (60181-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells.

  • IHC staining of human tonsillitis using 60181-1-Ig

    IHC staining of human tonsillitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human tonsillitis using 60181-1-Ig

    IHC staining of human tonsillitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IF Staining of human tonsillitis using 60181-1-Ig

    IF Staining of human tonsillitis using 60181-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green).

  • IF Staining of human tonsillitis using 60181-1-Ig

    IF Staining of human tonsillitis using 60181-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green).

  • IF Staining of Jurkat using 60181-1-Ig

    IF Staining of Jurkat using 60181-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60181-1-Ig

    IHC staining of human tonsillitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 60181-1-Ig

    IHC staining of human appendicitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 60181-1-Ig

    IHC staining of human liver using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60181-1-Ig

    IHC staining of human tonsillitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 60181-1-Ig

    IHC staining of human breast cancer using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human breast cancer using 60181-1-Ig

    IHC staining of human breast cancer using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human tonsillitis using 60181-1-Ig

    IHC staining of human tonsillitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human appendicitis using 60181-1-Ig

    IHC staining of human appendicitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human tonsillitis using 60181-1-Ig

    IHC staining of human tonsillitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human liver using 60181-1-Ig

    IHC staining of human liver using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human appendicitis using 60181-1-Ig

    IHC staining of human appendicitis using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human lymphoma using 60181-1-Ig

    IHC staining of human lymphoma using 60181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IF Staining of human tonsillitis using 60181-1-Ig

    IF Staining of human tonsillitis using 60181-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3), FOXP3 antibody (22228-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CD3 Monoclonal antibody

CD3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 60181-1-Ig

产品说明书

CloneNo. 3F3A1
引用文献(61)

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

别名

CD3E, 3F3A1, CD3 epsilon, CD3-epsilon, T3E

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMOLT-4 cells, Jurkat cells, human PBMCs
Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3200-1:12800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60181-1-Ig targets CD3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag11797

Product name: Recombinant human CD3E protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-207 aa of BC049847

Sequence: MQSGTHWRVLGLCLLSVGVWGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMDVMSVATIVIVDICITGGLLLLVYYWSKNRKAKAKPVTRGAGAGGRQRGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRRI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD3e molecule, epsilon (CD3-TCR complex)
别名 CD3E, 3F3A1, CD3 epsilon, CD3-epsilon, T3E
计算分子量 207 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC049847
基因名称 CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
RRIDAB_10859262
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07766
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.

实验方案

Product Specific Protocols
IF protocol for CD3 antibody 60181-1-IgDownload protocol
IHC protocol for CD3 antibody 60181-1-IgDownload protocol
WB protocol for CD3 antibody 60181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC,IF

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J Med Virol

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EBioMedicine

Patient-derived melanoma organoid models facilitate the assessment of immunotherapies

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IHC

Autophagy

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.

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Pharmacol Res

Mesothelin CAR-T cells expressing tumor-targeted immunocytokine IL-12 yield durable efficacy and fewer side effects

Authors - Yuankui Zhu
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humanIF

Sci Signal

ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis

Authors - Hao Cai
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