• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60323-1-Ig (same clone as 60323-1-PBS)

    WB analysis using 60323-1-Ig (same clone as 60323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 60323-1-Ig (same clone as 60323-1-PBS)

    IHC staining of human placenta using 60323-1-Ig (same clone as 60323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60323-1-Ig (same clone as 60323-1-PBS)

    IF Staining of HeLa using 60323-1-Ig (same clone as 60323-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60323-1-Ig (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

CD39/ENTPD1 Monoclonal antibody, PBS Only

CD39/ENTPD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60323-1-PBS

产品说明书

CloneNo. 2D2H6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ENTPD1, 2D2H6, ATP diphosphohydrolase, ATPDase, ATP-DPH

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

60323-1-PBS targets CD39/ENTPD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CD39/ENTPD1 fusion protein Ag6165 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ectonucleoside triphosphate diphosphohydrolase 1
别名 ENTPD1, 2D2H6, ATP diphosphohydrolase, ATPDase, ATP-DPH
计算分子量 58 kDa
观测分子量 54 kDa
GenBank蛋白编号BC047664
基因名称 CD39
Gene ID (NCBI) 953
RRIDAB_2881434
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49961
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing. The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 60323-1-Ig can recognize the soluble form of CD39 (38-478aa) molecule.